Tris(2-carboxyethyl)phosphine hydrochloride
TCEP is a water-soluble reducing agent commonly used to reduce disulfide bonds in proteins and peptides, both in vitro and in vivo. It is more stable than DTT and can also be used in mass spectrometry applications. Other uses include:
Reduction of sulfoxides, sulfonyl chlorides, N-oxides, and azides
Tissue homogenization process for RNA isolation
A chelating agent for heavy metal ions such as Zn(II), Cd(II), Pb(II), and Ni(II)
Labeling of cysteine residues with maleimides