Firefly Luciferase Assays are one of the leading reporter assays in the world in the measurement of gene function and gene regulation as well as being widely used in pharmaceutical screening. The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue. Firefly luciferase is a monomeric 62 kDa protein typically isolated from the firefly, Photinus pyralis, which catalyzes the ATP-dependent D-luciferin in the presence of oxygen and Mg2+ to oxyluciferin producing a yellow to greenish light (~560 nm).
GoldBio’s Illumination™ Lyophilized Firefly Luciferase Enhanced Assay Kit is specifically designed for the simple and efficient quantification of firefly luciferase reporter enzyme activity from cultured cells with the highest degree of sensitivity, reliability and linearity. In addition, the Firefly Luciferase Assay Buffer is lyophilized for more convenient shipping and storage.
This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.