Description
ROTI®GelStain Red Eco 10 000x conc., ready-to-use
ROTI®GelStain Red Eco is a versatile staining reagent for the detection of all nucleic acids in agarose and polyacrylamide gels.
Properties:
Red fluorescence
Alternative to ethidium bromide, non-toxic, non-mutagenic
For staining of dsDNA, ssDNA and RNA
Twice as sensitive as ethidium bromide (approx. 0.1 ng /band)
Can be used with the common ethidium bromide filters
Excitation with UV light (300 nm) and blue light (500 nm)
Compatible with all common downstream applications
ROTI®GelStain Red Eco is a red fluorescent dye which, like ethidium bromide, can be used to stain nucleic acids. For this purpose, the dye can either be added directly to the gel solution or used for post-run staining.
With the Ames test, it could be proven that ROTI®GelStain Red Ecois significantly less toxic than ethidium bromide. At the same time, the dye is twice as sensitive as ethidium bromide (approx. 0.1 ng/band). The red fluorescence of the dye can be documented with all common ethidium bromide photofilters.
ROTI®GelStain Red Eco is compatible with all common downstream applications.
Advantages:
More sensitive than ethidium bromide and ROTI®GelStain Red (0984.1)
Dissolved in water instead of DMSO
Also suitable for post-run staining
Wavelength
Excitation maximum(bound to DNA): approx. 300 nm and 500 nm
Emission maximum (bound to DNA): 600 nm.
Details
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